Development and Validation by UV Spectrophotometric Method for Simultaneous Estimation of Gliclazide and Quercetin in Formulation

Abstract

A simple, quick, accurate, precise, and cost-effective spectrophotometric approach for simultaneous estimation of Gliclazide and Quercetin has been developed by Simultaneous equation method. Gliclazide and Quercetin shows absorbance maximum at 226 and 255 nm respectively, as a result absorbance was measured at these wavelengths for the estimation of Gliclazide and Quercetin. In concentration ranges from 2-18 g/ml, and 2-22 g/ml for Gliclazide and Quercetin respectively followed Beer-Lambert\'s law. The method has been developed and validated in accordance with ICH recommendations and can be used for estimation of Gliclazide and Quercetin in formulations.

Introduction

IV. ACKNOWLEDGEMENT

The authors are thankful to Ideal cures and Ozone Labs, for providing drug samples and also to the Principal of H.K College of Pharmacy for providing facilities to carry out this research work.

V. CONFLICT OF INTEREST

The authors report no conflicts of interest.

Conclusion

The developed method was found to be sensitive, accurate, precise, reproducible and linear over the concentration range studied. Spectrophotometric method of analysis is more economical and easier, compared to methods such as chromatography and electrophoresis. The proposed method can be used for the routine quality control analysis of Gliclazide and Quercetin pharmaceutical dosage forms.

References

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Copyright

Copyright © 2023 Malissa Dmello, Dr. Anagha Raut, Dr. Geeta Bhagwat. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.